Cell Technology (CELL)5 ECTS
(englische Bezeichnung: Cell Technology)
(Prüfungsordnungsmodul: Cell Technology)

Lectures:

• • Cell Technology (Lecture)
    (Vorlesung, Christoph Lindenberger, FAU Busan only)
  • Cell Technology (Class)
    (Übung, Christoph Lindenberger, FAU Busan only)
  • Cell Technology (Practical)
    (Praktikum, Christoph Lindenberger, FAU Busan only)

Inhalt:

• What is meant by a sterile working environment in cell culture techniques (equipment, behaviour, safety precautions techniques)

• relevant culture systems (T-Flask and bioreactor; interactions with the cells, products, and nutrients)

• production of antibodies with hybridoma cells, insect cells and baculoviruses (biopesticides, recombinant proteins)

• immobilization (procedure, materials, xenogeneic transplants, immune protection, maximizing the yield of recombinant proteins)

• establishment of cell lines

• characterization of cell cultures (microscopy, trypan blue, MTT, resazurin, EZ4U in CFDA-SE, PJ, FACS, MACS)

• typical metabolic pathways in cell cultures

• needs of cell cultures (nutrients, pO2, adherence, shear stress, buffer systems, pH)

• medically relevant applications (liver replacement systems, cancer therapy, artificial skin)

• toxicity and toxicity tests

• stem cell procedures and cell differentiation

Lernziele und Kompetenzen:

The students should understand how to read professional literature critically. Based on 1-3 literature examples per teaching session, it will be shown how unreliable publications in respected journals can be. Furthermore, students learn how to produce the ideal culture system (for example, organ cultures) or how to optimize the yield of a process (e.g. antibodies, recombinant proteins) by identifying the relevant analogies with the living system and how to implement them practically. This includes an in-depth understanding of verifying analysis, biochemistry and material sciences.

Weitere Informationen:

Verwendbarkeit des Moduls / Einpassung in den Musterstudienplan:

1. Chemie- und Bioingenieurwesen (Master of Science)
   (Po-Vers. 2015w | TechFak | Chemie- und Bioingenieurwesen (Master of Science) | Masterprüfung | 1.-2. Wahlpflichtmodul (ohne Praktikum) | Cell Technology)

Studien-/Prüfungsleistungen:

Prüfungsleistung zu Cell Technology (Prüfungsnummer: 79101)

(englischer Titel: Examination Achievement: Cell Technology)

Untertitel: nur Busan

(englischer Untertitel: Busan only)

Prüfungsleistung, mündliche Prüfung, Dauer (in Minuten): 30, benotet

Anteil an der Berechnung der Modulnote: 100.0 %

Prüfungssprache: Englisch

Erstablegung: WS 2018/2019, 1. Wdh.: SS 2019, 2. Wdh.: WS 2019/2020

1. Prüfer: