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Chemistry of life (CE10/MSM-ME6)15 ECTS (englische Bezeichnung: Chemistry of life)
(Prüfungsordnungsmodul: Wahlpflichtmodul Molecular Science)
Modulverantwortliche/r: Ivana Ivanovic-Burmazovic Lehrende:
Andriy Mokhir, Ivana Ivanovic-Burmazovic, Marcus Fischer
Start semester: |
WS 2015/2016 | Duration: |
2 semester | Cycle: |
halbjährlich (WS+SS) |
Präsenzzeit: |
225 Std. | Eigenstudium: |
225 Std. | Language: |
Englisch |
Lectures:
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- A. Chemistry of Biomolecules and Cellular Functions (2 SWS/VORL)
- B. Cell Signaling and chemistry of oxidative stress (2 SWS/VORL)
- C. Seminar: Experimental Techniques and Selected Topics in Chemical Biology of Diseases (1SWS/SEM)
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Experimental Techniques and Selected Topics in Chemical Biology of Diseases (part1) (WS 2015/2016)
(Seminar, 1 SWS, Ivana Ivanovic-Burmazovic et al., Thu, 11:45 - 12:30, A00.14; second part of the seminar in the summer term)
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Experimental Techniques and Selected Topics in Chemical Biology of Diseases (part2) (SS 2016)
(Seminar, 1 SWS, Ivana Ivanovic-Burmazovic et al., Thu, 11:45 - 12:30, A 1.42 Egerlandstr.1; second half of the seminar in SS14)
- D . Practical session (10SWS/PRA)
Empfohlene Voraussetzungen:
B.Sc. in Chemistry / B.Sc. Molecular Science
(or equivalent molecule/synthesis-based studies)
Inhalt:
A.
Relation between the 3D protein structure and their function, including discussion of state-of-the-art methodology
Biochemistry of biological membranes (chemistry of carbohydrates, lipids an protein channels)
Chemical biology of nucleic acids as therapeutic targets
Protein-nucleic acid interactions
Cell as self-sustainable and bio-functional confined space
Nucleosides and Nucleotides
Chemical and biological synthesis of nucleic acids
DNA and RNA structure
Nucleic acids in biotechnology
Spectroscopic and structural methods applied in studies of nucleic acids
Nucleic acids and their analogues as drugs
B.
Bioinorganic chemistry of electron transfer
Thermodynamics and kinetics of mitochondrial processes
Biochemistry of free radical generation and removal
Monitoring oxidative stress in living systems
Interplay between oxidative stress and cell signaling pathways: Inflammation, Neuropathie and Cancer as model systems
Redox drugs
C. Seminar:
Preparation for practical session with an accent on the methodological approach and medical aspects D.
D1: Structure and function of proteins: denaturation/renaturation, protein-ligand interactions (UV-Vis, fluorescence and EPR spectroscopy, amperometric analysis of small-molecule interactions)
D2:
Kinetics of SOD and Catalase activity of natural enzymes and their
synthetic mimics (direct stopped-flow methods vs. indirect assays)
D3:
Cell preparation for fluorescence microscopy (staining of cell compartments and detection of oxidative stress)
D4:
Protein purification and analysis (cell extract preparation, 2D-electrophoresis of total cell extract, trypsin digestion, HPLC
separation and ESI-MS-detection)
D5:
Synthesis of a representative nucleic acid analogue; its identification (MALDI-TOF MS), purification (HPLC), quantification
(UV-visible spectroscopy), study of binding to a target nucleic acid (fluorescence spectroscopy, melting profile measurement).
D6:
As a DEMO-Experiment: testing inhibitors in cellular cultures, monitoring inhibition by using flow cytometry and RT PCR
Lernziele und Kompetenzen:
The main goal is to provide students with the up-to-date practical and operative know-how suitable for future scientific and/or applied work in research institutes, pharmaceutical/food industry, medical care laboratories, bio-technological, bio-analytical and environmental branches, accompanied by advanced theoretical background and overview of emerging trends in life sciences (chemistry, biology and medicine).
Rather than traditionally used phenomenological approach the courses are based on viewing living systems through the lens of basic chemical principles. The modern aspects of this module prepare students to work in interdisciplinary environment and participate in national and international development of forefront fields such as translational medicine.
Literatur:
Selected chapters from:
Voet & Voet, Biochemistry, Wiley & Sons;
Barry Halliwell & John M. C. Gutteridge, Free Radicals in Biology and medicine, Oxford
I. M. Rosenberg, Protein Analysis and Purification, Birkhäuser
Bertini, Gray, Stiefe, Valentine, Biological Inorganic Chemistry, Structure & Reactivity, University Science Books
F. Marks, U. Klingmüller, K. Müller-Decker, Cellular Signal Processing: An Introduction to the Molecular Mechanisms of Signal Transduction, Taylor & Francis
Bemerkung:
Courses of study for which the module is acceptable:
M.Sc. Molecular (Life) Science (Mandatory elective module/ Wahlpflichtmodul)/ M.Sc. Chemistry (Elective Module/Wahlmodul)
Organisatorisches:
Frequency of offer: Annually, starting WS; the module can only be taken as a whole
Verwendbarkeit des Moduls / Einpassung in den Musterstudienplan:
- Molecular Science (Master of Science)
(Po-Vers. 2013 | Wahlpflichtmodul Molecular Science)
Dieses Modul ist daneben auch in den Studienfächern "Chemie (Master of Science)" verwendbar. Details
Studien-/Prüfungsleistungen:
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