The creation of histological slices is a common approach to investigate tissue and tissue changes on a cellular basis. Sometimes, however, it would be beneficial if the underlying three-dimensional structure might be viewed as well, for example to be better able to identify morphological characteristics.

The aim of this project is the 3-D reconstruction of a set of histological slices, which includes investigation and implementation of the following steps:

• Selection of slices suitable for reconstruction

• Reordering slices to restore the proper sequence

• Artifact reduction

• Rigid and non-rigid registration of the slices

• Segmentation of Regions of Interest

• Saving and displaying a 3-D volume

This project is primarily focused on the reconstruction of the optical nerve head. It is of special interest in the case of eye diseases like glaucoma. By reconstruction, structural changes in that region should be perceived and investigated faster and easier.

Furthermore, the possible benefits of a combination of histological reconstructions with acquired volumes of other imaging modalities, e.g., OCT imaging, should be investigated.