Methods of modern (Confocal-) light microscopy (ILS-MA-B10)5 ECTS
(englische Bezeichnung: Methoden der moderen (Konfokalen-)Lichtmikroskopie)
(Prüfungsordnungsmodul: Methods of Modern (Confokal-) Light Microscopy)

Lectures:

• • Methoden der modernen Lichtmikroskopie
    (Übung, 6 SWS, Ruth Stadler, Blockveranstaltung 19.09. - 14.10.2016)

Empfohlene Voraussetzungen:

none

Inhalt:

Theoretical and practical introduction to following topics:

• Cloning of XFP-fusion constructs

• Transformation of model plants and cell systems

• Expression and localization experiments using different fluorescent proteins and dyes

• Protein interaction and dynamics (photoactivation; photoconversion, FRAP, BiFC)

• Genetic and pharmacological inhibition of cell biological processes

• Immunofluorescence
  

Lernziele und Kompetenzen:

The students will:

• get an overview of (confocal) microscopy techniques and the application of different fluorescent proteins as well as dyes in modern cell and molecular biology.

• critically discuss and evaluate publications addressing cell biological and plant physiological questions by employing microscopy techniques

• get a hands on training in molecular biological techniques and confocal laser microscopy using various imaging methods.
  

Literatur:

Will be provided 2-3 weeks prior to the seminar

Verwendbarkeit des Moduls / Einpassung in den Musterstudienplan:

1. Integrated Life Sciences: Biology, Biomathematics, Biophysics (Master of Science)
   (Po-Vers. 2015w | NatFak | Integrated Life Sciences: Biology, Biomathematics, Biophysics (Master of Science) | Module Groups | MG2: Bioimaging and Biophysics | Mandatory Elective Modules Group 2 | Methods of Modern (Confokal-) Light Microscopy)

Studien-/Prüfungsleistungen:

Portfolio exam: Methods of Modern (Confokal-) Light Microscopy (Prüfungsnummer: 83311)

Prüfungsleistung, mehrteilige Prüfung, benotet, 5 ECTS

Anteil an der Berechnung der Modulnote: 100.0 %

weitere Erläuterungen:
PL: Klausur 60 Min. SL: mündliche Präsentation 30 Min.

Erstablegung: SS 2019, 1. Wdh.: WS 2019/2020

1. Prüfer:

Benedikt Kost