Chemistry of life (CE10/MSM-ME6)15 ECTS
(englische Bezeichnung: Chemistry of life)

Lehrveranstaltungen:

• A. Chemistry of Biomolecules and Cellular Functions (2 SWS/VORL)
  • Chemistry of Biomolecules and Cellular Functions (WS 2015/2016)
    (Vorlesung, Andriy Mokhir, Do, 10:00 - 11:30, A00.14; starts at 29th October 2015)
• B. Cell Signaling and chemistry of oxidative stress (2 SWS/VORL)
  • Cell Signaling and chemistry of oxidative stress (SS 2016)
    (Vorlesung, 2 SWS, Ivana Ivanovic-Burmazovic et al., Do; vom 06.06.-09.06.2016 im Raum A00.12 und am 10.06.2016 im Raum A1.42 jeweils zwischen 9:00-13:00 Uhr, und vom 12.06.-15.06. zwischen 9:00 Uhr und 13:00 Uhr im Raum A00.12)
• C. Seminar: Experimental Techniques and Selected Topics in Chemical Biology of Diseases (1SWS/SEM)
  • Experimental Techniques and Selected Topics in Chemical Biology of Diseases (part1) (WS 2015/2016)
    (Seminar, 1 SWS, Ivana Ivanovic-Burmazovic et al., Do, 11:45 - 12:30, A00.14; second part of the seminar in the summer term)
  • Experimental Techniques and Selected Topics in Chemical Biology of Diseases (part2) (SS 2016)
    (Seminar, 1 SWS, Ivana Ivanovic-Burmazovic et al., Do, 11:45 - 12:30, A 1.42 Egerlandstr.1; second half of the seminar in SS14)
• D . Practical session (10SWS/PRA)
  • Chemistry of Life - Practical session (WS 2015/2016)
    (Praktikum, Ivana Ivanovic-Burmazovic et al., registration: contact Dr. Filipovic (milos.filipovic@fau.de) latest till 19th of October 2015, 3 weeks fulltime from 09.11.-29.11.2015 (lectures and seminars of the mandatory modules may be attended))
  • Chemistry of Life - Practical session (SS 2016)
    (Praktikum, Ivana Ivanovic-Burmazovic et al., this second part of the lab course will take place in may)

Empfohlene Voraussetzungen:

B.Sc. in Chemistry / B.Sc. Molecular Science (or equivalent molecule/synthesis-based studies)

Inhalt:

A.

• Relation between the 3D protein structure and their function, including discussion of state-of-the-art methodology

• Biochemistry of biological membranes (chemistry of carbohydrates, lipids an protein channels)

• Chemical biology of nucleic acids as therapeutic targets

• Protein-nucleic acid interactions

• Cell as self-sustainable and bio-functional confined space

• Nucleosides and Nucleotides

• Chemical and biological synthesis of nucleic acids

• DNA and RNA structure

• Nucleic acids in biotechnology

• Spectroscopic and structural methods applied in studies of nucleic acids

• Nucleic acids and their analogues as drugs

B.

• Bioinorganic chemistry of electron transfer

• Thermodynamics and kinetics of mitochondrial processes

• Biochemistry of free radical generation and removal

• Monitoring oxidative stress in living systems

• Interplay between oxidative stress and cell signaling pathways: Inflammation, Neuropathie and Cancer as model systems

• Redox drugs

C. Seminar: Preparation for practical session with an accent on the methodological approach and medical aspects

D.
D1: Structure and function of proteins: denaturation/renaturation, protein-ligand interactions (UV-Vis, fluorescence and EPR spectroscopy, amperometric analysis of small-molecule interactions)
D2: Kinetics of SOD and Catalase activity of natural enzymes and their synthetic mimics (direct stopped-flow methods vs. indirect assays)
D3: Cell preparation for fluorescence microscopy (staining of cell compartments and detection of oxidative stress)
D4: Protein purification and analysis (cell extract preparation, 2D-electrophoresis of total cell extract, trypsin digestion, HPLC separation and ESI-MS-detection)
D5: Synthesis of a representative nucleic acid analogue; its identification (MALDI-TOF MS), purification (HPLC), quantification (UV-visible spectroscopy), study of binding to a target nucleic acid (fluorescence spectroscopy, melting profile measurement).
D6: As a DEMO-Experiment: testing inhibitors in cellular cultures, monitoring inhibition by using flow cytometry and RT PCR

Lernziele und Kompetenzen:

The main goal is to provide students with the up-to-date practical and operative know-how suitable for future scientific and/or applied work in research institutes, pharmaceutical/food industry, medical care laboratories, bio-technological, bio-analytical and environmental branches, accompanied by advanced theoretical background and overview of emerging trends in life sciences (chemistry, biology and medicine).
Rather than traditionally used phenomenological approach the courses are based on viewing living systems through the lens of basic chemical principles. The modern aspects of this module prepare students to work in interdisciplinary environment and participate in national and international development of forefront fields such as translational medicine.

Literatur:

Selected chapters from:

• Voet & Voet, Biochemistry, Wiley & Sons;

• Barry Halliwell & John M. C. Gutteridge, Free Radicals in Biology and medicine, Oxford

• I. M. Rosenberg, Protein Analysis and Purification, Birkhäuser

• Bertini, Gray, Stiefe, Valentine, Biological Inorganic Chemistry, Structure & Reactivity, University Science Books

• F. Marks, U. Klingmüller, K. Müller-Decker, Cellular Signal Processing: An Introduction to the Molecular Mechanisms of Signal Transduction, Taylor & Francis

Bemerkung:

Courses of study for which the module is acceptable: M.Sc. Molecular (Life) Science (Mandatory elective module/ Wahlpflichtmodul)/ M.Sc. Chemistry (Elective Module/Wahlmodul)

Organisatorisches:

Frequency of offer: Annually, starting WS; the module can only be taken as a whole

Verwendbarkeit des Moduls / Einpassung in den Musterstudienplan:
Das Modul ist im Kontext der folgenden Studienfächer/Vertiefungsrichtungen verwendbar:

1. Chemie (Master of Science)
   (Po-Vers. 2009 | Wahlmodul | Chemistry of Life)
2. Molecular Science (Master of Science)
   (Po-Vers. 2013 | Wahlpflichtmodul Molecular Science)

Studien-/Prüfungsleistungen:

Chemistry of Life (Prüfungsnummer: 67501)

(englischer Titel: Chemistry of Life)

Prüfungsleistung, mündliche Prüfung, Dauer (in Minuten): 45, benotet

Anteil an der Berechnung der Modulnote: 100.0 %

weitere Erläuterungen:
Assessment and examinations: Lab course protocol(s) without marks + oral examination (45 min) 2 Examiners
Calculation of the grade for the module: 100% from oral examination

Prüfungssprache: Englisch

Erstablegung: SS 2016, 1. Wdh.: WS 2016/2017

1. Prüfer:

Ivana Ivanovic-Burmazovic